Cytotoxicity of Ethanol Extracts of in vivo, in vitro and Biotized Grown Plants of Vernonia divergens on EAC Cell Lines

The present study was attempted to enhance the anticancer activities in in vitro grown medicinal plant, Vernonia divergens, generated under the stress of a fungus, Piriformospora indica and to evaluate the effect of such biotization against EAC cell lines using standard MTT assay. The effect of adenine sulphate in addition to the phytohormones was assessed for micropropagation of V. divergens from shoot tip explants. The regenerated plantlets were biotized with a fungus, P. indica through soil drenching method. After acclimatization of 30 days, the ethanol extracts of these regenerants were screened for their inhibitory action against EAC mouse cell lines at different concentrations for 72 hours of treatment and compared with that of the inhibitory action of both in vivo and in vitro (non-biotized) generated plants. The combination of adenine sulphate and phytohormones sufficiently increased the number of shoots and roots per explants. Biotization of micropropagated plantlets not only enhanced the survival rate and growth of root, shoot and leaf of the regenerated plants but it also increased the anticancer potentiality. The level of inhibition of ethanol extract against EAC cell lines of these regenerated plants was found to be low as compared with that of the natural garden plants but the stress of biotization with this fungus helped the regenerants to regain the inhibitory action against the cell lines. Thus, biotization with the fungus, P. indica is vital not only in the growth promotion of plants but also demonstrated enhanced anticancer potentialities in micropropagated plants, grown under stress.